Blood Biochemical Changes Associated with Acute Treatment of Orchis Anatolica Plant Roots Ethanol Extract in Adult Albino Rats

We explore through this study blood parameters changes after 7 days treatment with Orchis anatolica (O. anatolica) plant roots ethanol extract in Albino rats. An intra-peritoneal glucose tolerance test (IPGTT) was carried on to evaluate the hypoglycemic effect of the extract on blood glucose and insulin titer using Albino rats fasting for 18 hours. Blood glucose and serum insulin levels where determined throughout one hour period prior to a single treatment dose of 400mg/kg/BW O. anatolica extract. Oral treatment with O. anatolica was then performed using another group of 10 Albin rats treated with 400mg/kg/BW/day for 7 days and where compared with a control group. Using UV/Visible spectrophotometer, glucose, cardiac and lever serum blood biochemical markers were investigated in addition to blood total protein urea. Significant reduction in cardiac markers namely serum cholesterol (LDL) and Creatinin Kinase (KC) titers were detected after the treatment. Alanine AminoTransferase (ALT), Aspartate Amino Transferase (AST and) ad, triglycerides representing hepatic markers were normal concomitant with normalized values of bilirubin, total protein and blood urea when compared with the control. Blood glucose levels were reduced to significant levels after 7 days treatment with the plant extract an observation that was further demonstrated by IPGTT. Concomitant, an increase in serum insulin level was observed also when IPGTT was performed. We can conclude that O. anatolica root extract induces hypoglycemia, insulin-releasing and cholesterol lowering effects in rats. Together, a normal biochemical marker indicates an organ protective ability of the plant.